|Overhead view of a subnanometer resolution reconstruction showing the microtubule-binding region of the Ndc80 complex (blue) attached to a microtubule(green). The part of the Ndc80 complex away from the microtubule is flexible and appears as scattered density in the reconstruction (red). (Image courtesy of Nogales group)|
"Our model suggests that Ndc80 oligomerizes on the surface of the microtubule via a segment of the protein that is regulated so that correct attachments are maintained and incorrect attachments are discarded," says biophysicist Eva Nogales who led this study.
"What we propose is that this oligomerization is an important part of the mechanism by which Ndc80 is able to utilize the energy of microtubule disassembly to move chromosomes towards the spindle poles during mitosis. This oligomerization will only happen for correctly attached microtubules"
|A kinetochore is the multi-protein structure in which microtubule spindle fibers engage chromosomes to align and separate them equally between daughter cells during cell division.(Image courtesy of Nogales group)|
Co-authoring the paper with Nogales were Gregory Alushin, Vincent Ramey, Sebastiano Pasqualato, David Ball, Nikolaus Grigorieff and Andrea Musacchio.
) Biological cells have a cytoskeleton that gives shape to membrane walls and other cellular structures and also controls the transportation of substances in and out of the cell. This cytoskeleton is spun from tiny fibers of tubulin protein called microtubles. During mitosis, microtubules disassemble and reform into spindles across which duplicate sets of chromosomes line up and migrate to opposite poles. After chromosome migration is complete, the microtubules disassemble and reform back into skeletal systems for the two new daughter cells.
Mistakes in the distribution of chromosomes from a parent cell to its daughter cells can lead to birth defects, cancer and other disorders. To ensure that each daughter cell receives a single copy of each chromosome, microtubule spindles dock with each chromosome's centromere - the central region where its two chromatids connect. The microtubule spindles connect with the centromere through a network of proteins called the kinetochore. Ndc80 is a key member of the kinetochore network and serves as a sort of "landing pad" for the microtubule-centromere connection. Although Ndc80's genetics and biochemistry have been extensively characterized, the mechanisms behind its activities have until now remained a mystery.
"Our first ever subnanometer model of Ndc80 shows that the protein complex binds the microtubule with a tubulin monomer repeat that is sensitive to tubulin conformation," Nogales says. "Furthermore, Ndc80 complexes self-associate along microtubule protofilaments via interactions that are mediated by the amino-terminal tail of the Ndc80 protein, which is the site of phospho-regulation by the Aurora B kinase."
The Aurora B kinase is an enzyme that ensures the correction of any improper microtubule-kinetochore attachments - faulty attachments will result in unequal segregation of the genetic material, such as both chromatides going to the same daughter cell. In their paper, Nogales and her co-authors contend that Ndc80's mode of interaction with the microtubule and its oligomerization provide a means by which the Aurora B kinase can regulate the stability of the load-bearing Ndc80-microtubule attachments.
"The Aurora B kinase corrects wrong microtubule-kinetochore attachments by phosphorylating proteins in the kinetochore," Nogales says. "Ndc80 is a major substrate of this regulation. Our work shows that if phosphorylated by Aurora B, attachments are not robust because there is no oligomerization of Ndc80s."
This research was supported by a grant from the National Institute of General Medical Sciences.